Professor Takeshi Motohashi

Area and Subject Taught Plant Physiology and Biochemistry
Research Theme(s) Functional regulation in chloroplast of plants
Academic Degrees Ph. D. (Science)
Keywords for Research Field Plant Physiology, Plant Biochemistry
Office Phone Number Not Public
e-mail

Research Overview

Higher plants survive by greatly changing their metabolism, consisting of photosynthesis and accompanying carbon dioxide fixation, during the daytime when they are exposed to light and during the nighttime when they are not exposed to light. Chloroplasts are organelles that fulfi ll a very important role during plant photosynthesis and they are also necessary to allow a plant to adapt to the dramatic environmental changes that take place between nighttime and daytime. Our research seeks to uncover how these chloroplasts allow plants to adapt to the light environment changes that occur between daytime and nighttime and how they regulate the metabolic reactions within cells. This kind of control mechanism is generally called a redox regulation. In order to clearly understand the physiology and molecular mechanisms of the redox regulation of higher plant chloroplasts, we are using plant materials such as those of the genus Arabidopsis and employing various methods including physiological and biochemical methods in our research.

Notable Publications and Works in the Last Three Years

  1. Yuki Okegawa and Ken Motohashi "Expression of spinach ferredoxin-thioredoxin reductase using tandem T7 promoters and application of the purified protein for in vitro light-dependent thioredoxin-reduction system" Protein Expr. Purif. 121, 46-51 (2016)
  2. Yuki Okegawa, Masanori Koshino, Teruya Okushima and Ken Motohashi "Application of preparative disk gel electrophoresis for antigen purification from inclusion bodies" Protein Expr. Purif. 118, 77-82 (2016)
  3. Yuki Okegawa and Ken Motohashi "Chloroplastic thioredoxin m functions as a major regulator of Calvin cycle enzymes during photosynthesis in vivo" Plant J. 84, 900-913 (2015)
  4. Yuki Okegawa and Ken Motohashi "A simple and ultra-low cost homemade seamless ligation cloning extract (SLiCE) as an alternative to a commercially available seamless DNA cloning kit" Biochem. Biophys. Rep. 4, 148-151 (2015)
  5. Yuki Okegawa and Ken Motohashi "Evaluation of seamless ligation cloning extract (SLiCE) preparation methods from an Escherichia coli laboratory strain" Anal. Biochem. 486, 51-53 (2015)
  6. Ken Motohashi "A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis" BMC Biotechnol. 15, 47 (2015)
  7. Ken Motohashi and Yuki Okegawa "Method for enhancement of plant redox-related protein expression and its application for in vitro reduction of chloroplastic thioredoxins" Protein Expr. Purif. 101, 152-156 (2014)