Associate Professor Shinobu Chiba
|Area and Subject Taught||Molecular Biology|
|Research Theme(s)||Molecular biology of protein biogenesis and localization|
|Keywords for Research Field||Protein biogenesis, Protein localization, Translation|
|Office Phone Number||Not Public|
All living organisms synthesize proteins according to their own genetic information encoded by the DNA molecules. Since biological activities in the cells depend on a series of biochemical reactions catalyzed generally by proteins, protein synthesis, which is also referred to as “translation”, is namely a key process to generate biological functions from the blueprint of life. We are interested in and studying on a novel aspect of translation where newly synthesized proteins, or nascent polypeptides sometimes cotranslationally interact with the ribosome and thereby regulate the fates and births of their own. Increasing examples of such kinds of proteins that function during being synthesized is extending our knowledge as to how genetic codes are translated into biological functions. Through our research activities, we would like to understand still hidden principles of the central dogma of gene expression, where nascent polypeptides may play key roles.
Notable Publications and Works in the Last Three Years
- Chadani, Y., Niwa, T., Izumi, T., Sugata, N., Nagao, A., Suzuki, T., Chiba, S., Ito, K. and Taguchi, H. (2017) Intrinsic ribosome destabilization underlies translation and provides an organism with a strategy of environmental sensing. Mol Cell. 68, 528-539.e5.
- Chadani, Y., Niwa, T., Chiba, S., Taguchi, H. and Ito, K. (2016) Integrated in vivo and in vitro nascent chain profiling reveals widespread translational pausing. Proc. Natl. Acad. Sci. USA. 113, E829-E838.
- Ishii, E., Chiba, S., Hashimoto, N., Kojima, S., Homma, M., Ito, K., Akiyama, Y. and Mori, H. (2015) Nascent chain-monitored remodeling of the Sec machinery for salinity adaptation of marine bacteria. Proc. Natl. Acad. Sci. USA. 112, E5513-E5522.
- Sohmen, D., Chiba, S., Shimokawa-Chiba, N., Innis, A., Berninghausen, O., Beckmann, R., Ito, K. and Wilson, D. (2015) Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling. Nat. Commun. 6, 6941.
- Shimokawa-Chiba, N., Kumazaki, K., Tsukazaki, T., Nureki, O., Ito, K. and Chiba, S. (2015) Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein. Proc. Natl. Acad. Sci. USA. 112, 5063-5068.
- *Chiba, S. and Ito, K. (2015) MifM monitors total YidC activities of Bacillus subtilis including that of YidC2, the target of regulation. J Bacteriol. 197, 99-107.
(* corresponding author)