Professor Hideaki Tsuge

Area and Subject Taught Protein Structural Biology
Research Theme(s) Structural Biology of infectious disease
Academic Degrees ph.D(Faculty of Sciences)
Keywords for Research Field Protein Crystallography,Structure,Infectious Disease
Office Phone Number 81-75-705-3117

Research Overview

We are currently pursuing the structural biology of infectious disease. I am interested in the protein-protein interactions between infectious factors and host protein. Now we are studying the next two main themes. (1) In 2008, we revealed the complex crystal structure of actin specifi c ADP-ribosylating toxin (ADPRT) and host protein actin. Up to that time, many toxin structures were available, but there was no complex structure at all. Our structure provides the clue of ADP-ribosylation reaction between protein and protein. Now we are focusing RhoA specifi c ADPRT and RhoA interaction. (2) In highly pathogenic infl uenza, some mutations in RNA polymerase are known. Especially E627K mutant is very famous. In 2009, we revealed the crystal structure of PB2 C-terminal domain including K627. This structure provides the fi rst insight of the reason of highly pathogenicity. Furthermore we try to reveal the whole RNA-polymerase complex of infl uenza A virus.

Notable Publications and Works in the Last Three Years

  1. Tsurumura T, Qiu H. Tsumori Y, Oda M, Nagahama M, Sakurai J, Tsuge H : Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex. Proc Natl Acad Sci U.S.A. 110(11):4267-4272. 2013
  2. Yoshida T, Tsuge H, Hisabori T, Sugano Y. :Crystal structures of dye-decolorizing peroxidase with ascorbic acid and 2, 6-dimethoxyphenol. FEBS Lett. 586(24):4351-6. 2012
  3. Imagawa T, Tsurumura T, Sugimoto Y, Aki K, Ishidoh K, Kuramitsu S, Tsuge H. :Structural basis of the free reduced flavin generation by flavin reductase from Thermus thermophilus HB8. J. Biol. Chem. 286, 44078-44085. 2011