Visiting Professor Nobuo Shimamoto

Area and Subject Taught Nanobiology/Nanotechnology
Research Theme(s) Nanobiology of gene expression
Academic Degrees Doctor of Science, Kyoto University
Keywords for Research Field transcription, translation, bacteriology
Office Phone Number 81-75-705-3078

Research Overview

Nobuo Shimamoto, one of the people who started nanobiology in 1993, is now interested in the survival strategy of bacteria by using the newest tools of nano-manipulation in combination with genetics. E. coli has been believed to be a simple creature growing as isolated cells, and individual cells are believed to have the same characteristics. Cell division is supposed to be stopped in its stationary state. We are challenging to these conventional hypotheses by introducing new nano-techniques. In our single-molecule dynamics of translational cycle, we solved the order of dissociation of translational complex in termination, 50S first and then mRNA, the answer of long-standing argument. We also clarified the mechanism of hibernation of ribosome in stationary state. Our new SEM technique enabled us to detect the variety of shapes and clustering of cells, opening a new bacteriology of E. coli involving the cell-cell communication for adaptation to nutrient defi cient, cell diff erentiation, and new function of tmRNA.

Notable Publications and Works in the Last Three Years

  1. Shimamoto, N., (2013) Bacterial transcription in “Encyclopedia of Systems biology” ed. Werner Dubitzky, W, Wolkenhauer, O., Cho, K-H., and Yokota, H. Springer-Verlag GmbH, Heidelberg, in press
  2. Shcherbakova, K., Hatakeyama, A.,Amemiya, Y., and Shimamoto, N., (2012) Nanoindentation as a Tool to Clarify the Mechanism Causing Variable Stiffness of a Silane Layer on Diamond In Nanoindentation in Materials Science; Nemecek, J., Ed. InTech, 2012; pp. 161–178.
  3. Aramaki, H., Kabata, H., Takeda, S., Itou, H., Nakayama, H., and Shimamoto, N., (2011) Formation of repressor-inducer-operator ternary complex: Negative cooperativity of D-camphor binding to CamR. Genes. Cells 16 1200-7.
  4. Imashimizu M., Tanaka K., and Shimamoto, N. (2011) Comparative study of cyanobacterial and E. coli RNA polymerases: misincorporation, abortive transcription, and dependence on divalent cations. Genetics Res. Internat. 2011 Article ID 572689
  5. Geertz, M., Travers, A., Mehandziska, S., Sobetsko, P., Janga, S., Shimamoto, N., and Muskhelishvili, G. (2011) Structural coupling between RNA polymerase composition and DNA supercoiling in coordinating transcription: a global role for the omega subunit? Mbio 2 0034-11
  6. Shimamioto, N (2010) President of 11th Asian and Oceanian Conference on Transcription, Okinawa, Nakijin, Japan