Visiting Professor Kazuhiro Nagata

Area and Subject Taught Molecular and Cellular Biology
Research Theme(s) 1) Life of Proteins; Protein Quality Control Mechanism and Diseases
2) Regulation of Cellular Functions by Molecular Chaperones
Academic Degrees Ph.D. (Kyoto Univ., D. Sc.)
Keywords for Research Field TMolecular chaperones, protein quality control
Office Phone Number 81-75-705-3090

Research Overview

The most important player for vital activity is the protein. After translation, proteins should be correctly folded by the aid of various molecular chaperones, resulting in acquiring the correct conformation and the authentic functions. When cells suffer from various cellular stresses, proteins are misfolded, which often causes various neurodegenerative diseases including Alzheimer’s disease. Cells have acquired protective mechanisms for such adverse conditions, which is called as protein quality control (PQC) mechanism. We have found and cloned several key molecules involved in such PQC mechanism. We are proceeding the studies elucidating the molecular mechanism for the selective degradation of misfolded proteins in the cells. Based on the background of cell biology, we are performing our studies by adopting various technologies including biochemistry, molecular biology, developmental biology and structural biology. We stick our research almost solely on the proteins that we found and cloned by ourselves, which we expect to help us to provide the novel concepts on this fi eld.

Notable Publications and Works in the Last Three Years

  1. Morito, K. Nishikawa, J. Hoseki, A Kitamura, Y. Kotani, K. Kiso, M. Kinjo, Y. Fujiyoshi & K. Nagata
    Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state
    Scientific Reports in press
  2. T. Kakihana, K. Araki, S. Vavassori, S. Iemura, M. Cortini, C. Fagioli, T. Natsume, R. Sitia & K. Nagata
    Dynamic regulation of Ero1α and Prx4 localization in the secretory pathway
    J. Biol. Chem. 288(41):29586-29594(2013)
  3. R.Ushioda, J.Hoseki & K. Nagata
    Glycosylation-independent ERAD pathway serves as a backup system under ER stress
    Mol. Biol. Cell. 24(20):3155-3163(2013)
  4. K.Araki, S.Iemura, Y.Kamiya, D.Ron, K.Kato, T. Natsume & K. Nagata
    Ero1α and PDIs constitute a hierarchical electron transfer network of endoplasmic reticulum oxidoreductases
    J. Cell. Biol. 202(6):861-874(2013)
  5. M. Hagiwara and K. Nagata
    Redox-dependent protein quality control in the ER : folding to degrasation.
    Antioxidants & Redox Signaling  16(10):1119-1128(2012)
  6. T. Ono, T. Miyazaki, Y. Ishida, M. Uehata and K. Nagata: Direct in vitro and in vivo evidence for interaction between Hsp47 protein and collagen triple helix. J. Biol. Chem. 287(9):6810-6818(2012)
  7. Y. Masago, A. Hosoya, S. Kawano, A. Nasu, J. Toguchida, K. Fujita, H. Nakamura, G. Kondoh and K. Nagata Molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation. J. Cell Sci. 125(5):1118-1128(2012)